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human mgmt pcmv6  (OriGene)


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    Structured Review

    OriGene human mgmt pcmv6
    Human Mgmt Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mgmt+pcmv6/MGMT+(NM_002412)+Human+Tagged+ORF+Clone/pmc12056744-52-16-21
    Average 93 stars, based on 6 article reviews
    human mgmt pcmv6 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment
    Article Snippet: MGMT (Invitrogen, 35-7000, 1:500) and β-actin (Sigma, A2228, 1:10,000) primary antibodies were incubated at 25°C for 90 min, and HRP-conjugated anti-mouse secondary antibodies (Cell Signaling; 1:2000) were incubated for 60 min at 25°C. .. Cells were seeded in 6-well plates at 1 × 10^6 cells/well and transfected with 2.5 μg human MGMT-pCMV6 ( NM_002412 ) (Origene RC229131) or empty pCMV6-Entry Myc-DDK-tagged vector (Origene PS100001) using OptiMEM and Lipofectamine 3000 (Invitrogen, Waltham, MA) for 72 h. Overexpression was confirmed by western blot. .. Cells at approximately 60% confluence in 6-well plates were transfected with 25 nM ON-TARGETplus SMARTpool siRNA targeting human MGMT (Dharmacon, L-008856-01-0005) or ON-TARGETplus control non-targeting pool siRNA (Dharmacon, D-001810-10-05) in OptiMEM with DharmaFECT 1 for 48 h. MGMT knockdown was confirmed by western blot.

    Over Expression:

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment
    Article Snippet: MGMT (Invitrogen, 35-7000, 1:500) and β-actin (Sigma, A2228, 1:10,000) primary antibodies were incubated at 25°C for 90 min, and HRP-conjugated anti-mouse secondary antibodies (Cell Signaling; 1:2000) were incubated for 60 min at 25°C. .. Cells were seeded in 6-well plates at 1 × 10^6 cells/well and transfected with 2.5 μg human MGMT-pCMV6 ( NM_002412 ) (Origene RC229131) or empty pCMV6-Entry Myc-DDK-tagged vector (Origene PS100001) using OptiMEM and Lipofectamine 3000 (Invitrogen, Waltham, MA) for 72 h. Overexpression was confirmed by western blot. .. Cells at approximately 60% confluence in 6-well plates were transfected with 25 nM ON-TARGETplus SMARTpool siRNA targeting human MGMT (Dharmacon, L-008856-01-0005) or ON-TARGETplus control non-targeting pool siRNA (Dharmacon, D-001810-10-05) in OptiMEM with DharmaFECT 1 for 48 h. MGMT knockdown was confirmed by western blot.

    Western Blot:

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment
    Article Snippet: MGMT (Invitrogen, 35-7000, 1:500) and β-actin (Sigma, A2228, 1:10,000) primary antibodies were incubated at 25°C for 90 min, and HRP-conjugated anti-mouse secondary antibodies (Cell Signaling; 1:2000) were incubated for 60 min at 25°C. .. Cells were seeded in 6-well plates at 1 × 10^6 cells/well and transfected with 2.5 μg human MGMT-pCMV6 ( NM_002412 ) (Origene RC229131) or empty pCMV6-Entry Myc-DDK-tagged vector (Origene PS100001) using OptiMEM and Lipofectamine 3000 (Invitrogen, Waltham, MA) for 72 h. Overexpression was confirmed by western blot. .. Cells at approximately 60% confluence in 6-well plates were transfected with 25 nM ON-TARGETplus SMARTpool siRNA targeting human MGMT (Dharmacon, L-008856-01-0005) or ON-TARGETplus control non-targeting pool siRNA (Dharmacon, D-001810-10-05) in OptiMEM with DharmaFECT 1 for 48 h. MGMT knockdown was confirmed by western blot.



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    93
    OriGene human mgmt pcmv6
    Human Mgmt Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mgmt+pcmv6/MGMT+(NM_002412)+Human+Tagged+ORF+Clone/pmc12056744-52-16-21
    Average 93 stars, based on 1 article reviews
    human mgmt pcmv6 - by Bioz Stars, 2026-10
    93/100 stars
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